PCR amplicon sequencing enables cost-effective whole genome sequencing and characterisation of target genes, but these primer schemes lack standardised data formats, a problem highlighted during the COVID-19 pandemic. Here we describe a text-based specification using primer.bed and reference.fasta files to standardise primer scheme definitions and facilitate data exchange for synthesis, lab work, and bioinformatics.
This specification was designed as a community resource. Therefore, we strongly encourage community engagement and feedback.